Cosmid Pics

Because of their high capacity, they are ideal for building comprehensive libraries of an organism's entire genome. Shuttle Vectors: Some, like the pFD666 vector

The Allen Lab at Stanford (fictionalized example) spent three months failing to isolate a 40 kb insert for a CRISPR delivery vector. They kept obtaining 15 kb inserts. One glance at their cosmid pic — a restriction digest gel — showed an extra 2.8 kb band in every clone. That band matched the vector’s stuffer fragment. The problem? Incomplete digestion of the stuffer during library construction. The visual evidence allowed them to redesign their partial Sau3AI digestion protocol, and they succeeded on the next attempt. cosmid pics